| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Somerset College Prep | A | Barber | Bourne-Morgan | Lopez | Wright | DNS |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Treasure Coast Classical | A | Sullivan | Kisner | Loew | Prestia | 1:00.96 | MCHS Sarge Invitational 2025 |
| 2 | Tradition Prep | A | Parrel | Falcon | Derius | Delevery | 53.15 | Jensen Beach Invitational 2026 |
| 3 | Martin County |
A | Prusenko | Ramlagan | Meglis | Ebanks | 50.18 | FHSAA 4A Region 2 |
| 4 | Fort Pierce Westwood |
A | Villegas | Brown | Blaise | Tolbert | 49.45 | Bill Wilson Invitational 2025 |
| 5 | Legacy High School | A | Nevarez | Pierre | Hudnet | Townsley | NT | |
| 6 | South Fork |
A | Holmes | Padgett | Pepsidero | Marrone | 52.22 | Jensen Beach Invitational 2026 |
| 7 | Jensen Beach |
A | Melda | Peruski | Trimble | Laurent | 54.40 | Jensen Beach Invitational 2026 |
| 8 | St_Andrews_Episcopal |
A | Roccasecca | Courtemanche | Essoh | Hinote | 1:05.41 | Jensen Beach Invitational 2026 |