| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | South Fork |
A | Brinn | Spatara | Ensor | Robinson | 9:35.60 | FHSAA 3A Region 4 |
| 2 | Martin County |
A | Schwartz | Esser | Linda | Scott | 10:13.45 | FHSAA 4A District 8 |
| 3 | Jensen Beach |
A | Ford | Sheridan | Readling | Morris | 10:21.69 | FHSAA 3A Region 4 |
| 4 | Somerset College Prep | A | Stoller | Dimanche | Jean-Noel | Morales | 11:41.76 | Pahokee Andre Powell Invitational |
| 5 | John Carroll |
A | DUFFY | Tokman | Fulmer | Russell | DNS | Vero Beach HS 2026 Preseason Classic |
| 6 | Treasure Coast Classical | A | Meeker | Kisner | Apfel | Hook | 12:38.35 | Benjamin Invite #2 2025 (Sanctioned) |
| 7 | Legacy High School | A | Taylor | Heysquierdo | Jean | Alviene | NT |