| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | South Fork |
A | Christoff | Quintero | Szpregelewski | Venuti | DNS | Martin County Championships 2025 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Pine School |
A | Devaney | Sprague | Whitaker | Anderson | NT | |
| 2 | Clewiston |
A | mckenzie | mullings | Dixon | Givens | 3:39.85 | FHSAA 2A District 12 |
| 3 | Port St. Lucie |
A | Connelly | Wright | Tavarez | Fulton | 3:35.05 | MCHS Sarge Invitational 2025 |
| 4 | Dwyer |
A | Martinez | Quince | Williams | Wilson | 3:24.28 | FHSAA 4A Region 2 |
| 5 | Martin County |
A | Allison | St. Juste | Brown | McLean | 3:33.52 | FHSAA 4A Region 2 |
| 6 | John Carroll |
A | BARBER | JACKSON | RICHARDSON | Housing | 3:39.36 | FHSAA 1A Region 4 |
| 7 | Jensen Beach |
A | Avril | Parr | Phillips | Tell | 3:55.24 | FHSAA 3A District 13 |
| 8 | Tradition Prep | A | Monaco | Beausejour-Chester | Salomon | Lopez | 3:56.09 | Bulldog Invitational 2025 |