| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Martin County |
A | Lawson | Orellana | Saracina | Lopes | 8:16.17 | FHSAA 4A District 8 |
| 2 | Dwyer |
A | Jefferson | Kamaras | Correa | Purcell | 8:20.33 | FHSAA 4A District 8 |
| 3 | Jensen Beach |
A | Sanchez | Francisco-Perez | Gebus-Mockabee | Cessna | 8:42.57 | The Lincoln Knowles Invitational 2025 |
| 4 | Clewiston |
A | mullings | Exilhomme | Hernandez | Bussott | 9:06.80 | FHSAA 2A Region 3 |
| 5 | South Fork |
A | Shivola | McDermott | Adams | Christoff | 9:26.40 | Cobra Relays 2025 |
| 6 | Tradition Prep | A | Brogan | Demosthenes | Matos | Quintavalle | 9:28.30 | Atlantic HS Last Chance |
| 7 | Pine School |
A | Devaney | Whitaker | Sprague | Anderson | DNS | FHSAA 1A District 13 |
| 8 | Moore Haven |
A | Rico | Malagon | Rico | Alfonso | 9:43.65 | Sebring Relay Meet 2025 |
| 9 | John Carroll |
A | Schildwatcher | Perez | MAURICIO | ASHER | 9:47.20 | Vero Beach HS 2026 Preseason Classic |