| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | VILLEGAS, Logan | 1:33.24 | Spanish River HS Invitational Track and Field 2026 | |
| 4 | OLSEN, Aaliyah | American Heritage (DB) |
1:07.12 | John I Leonard Invitational 1 |
| 5 | SCHROEDER, Natascha | Wellington |
1:17.25 | The Lincoln Knowles Invitational 2025 |
| 6 | RAMSEY, Jayla | NT |