| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | TIBEBE, Ruth | Park Vista | NT | |
| 2 | SMART, Tevenese | Centennial | NT | |
| 3 | SAINTYL, Elsheema | Trinity Christian (LW) | 23.85 | Seminole Ridge Hawks Last Chance |
| 4 | ENAMORADO, Anayah | Atlantic Christian | 21.50 | Saint Andrew's HS Track Invitational #3 |
| 5 | POWELL, Hailey | Park Vista | 21.88 | MCHS Tiger Invitational 2023 |
| 6 | CASSEUS, Shelliah | Santaluces | NT | |
| 7 | BLANC, Eika | Inlet Grove | NT | |
| 8 | SIMEON, Patricia | Inlet Grove | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | CANELAS, Angelique | Atlantic Christian | 20.30 | |
| 2 | FOWLER, Ta'Niyah | Glades Central | 19.82 | Lincoln Knowles Memorial T&F Invitational Meet |
| 3 | PETIT-HOMME, Jalah | Lake Worth Christian | 19.23 | Panther Open |
| 4 | NUZZOLO, Katelyn | Seminole Ridge | 17.03 | Glades Central Raiders Invitational |
| 5 | RUIZ, Samara | West Boca Raton | 18.76 | West Boca Raton Invitational |
| 6 | MASSENA, Chrislaine | Park Vista | 19.45 | Spartan Invitational - Southridge Park |
| 7 | BUSH, Shamiyah | Glades Central | 20.27 | |
| 8 | WILLIAMS, Kamia | Glades Central | 21.42 |