| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Palm Beach Gardens | A | 3:44.77 | Dwyer Panther Relays |
| 2 | Somerset Canyons | A | DNS | Palm Beach County Non-Conference Qualifier |
| 3 | Martin County | A | 3:31.82 | FHSAA 4A District 10 |
| 4 | Fort Pierce Central | A | 3:28.21 | FHSAA 4A Region 3 |
| 5 | Vero Beach | A | 3:29.85 | FHSAA 4A District 10 |
| 6 | Jupiter | A | 3:38.87 | Jupiter Warrior Invitational (17th Annual) |
| 7 | Suncoast | A | 3:43.75 | Dwyer Invitational |
| 8 | Lake Worth | A | 3:55.00 |