| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | THOMPSON, Azariah | E.O.T.O. ELITE | NT | |
| 2 | MCNEAL, Ke'Laijah | Pahokee Elite Track | NT | |
| 3 | LIDDLE, Paige | 2Fast Track Club | NT | |
| 4 | FLOWER, Jennavecia | TCBlaze | NT | |
| 5 | HOWARD, Layla | Indian River Elite Track Club | NT | |
| 6 | LOVE, Ivyanna | Pahokee Elite Track | NT | |
| 7 | RANDOLPH, Liliana | Treasure Coast Trailblazers G | NT | |
| 8 | YOUNG, Tiana | Take Flight Athletics | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | DOVE, Emmanuella | Swiffers Track Club | NT | |
| 2 | WILLIAMS, Khamiah | Take Flight Athletics | 32.60 | |
| 3 | HARDY, Christiana | Power House Athletics Track & | 31.56 | |
| 4 | MCPHERSON, Valiya | QUANTUM TRACK CLUB | 30.34 | |
| 5 | MILLER, Jade | JAM RockStars | 31.02 | |
| 6 | MURRAY, Jada | Take Flight Athletics | 31.70 | |
| 7 | CLARK, Avah | Take Flight Athletics | 33.80 | |
| 8 | EWAR, Tivona | Indian River Elite Track Club | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | DICKENSON, Keviana | Swiffers Track Club | 29.14 | |
| 2 | SMITH, Ta'Naisha | Synergy Athletics | 29.00 | |
| 3 | MCPHERSON, NAHJIYA | QUANTUM TRACK CLUB | 28.82 | |
| 4 | WATKINS, Kharisma | Watkins Family United | 27.00 | |
| 5 | DEVOE, Camariya | E.O.T.O. ELITE | 28.00 | |
| 6 | EDMOND, Harmoni | Indian River Elite Track Club | 29.00 | |
| 7 | COUSINS, Shayenne | Swiffers Track Club | 29.11 | |
| 8 | PETTIGREW, Xolani | TCBlaze | 29.77 |