| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | WILLIAMS, Jacoby | Boca Warriors Track Club | NT | |
| 3 | JEAN, Patrick | TCBlaze | NT | |
| 4 | NANCE, Cody | QUANTUM TRACK CLUB | 41.13 | |
| 5 | KENNEDY, Mekhi | Indian River Elite Track Club | NT | |
| 6 | CRAYTON, Kyran | Synergy Athletics | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | PATTERSON, Messiah | 1804 Track Club | 39.10 | |
| 3 | JOHNSON, Johnathan | Pahokee Elite Track | 37.00 | |
| 4 | MCRAE, Ky'Shaud | Pahokee Elite Track | 36.00 | |
| 5 | STEPHENS, Jordan | Space Coast Striders Track Clu | 36.80 | |
| 6 | PATA, Joshua | 1804 Track Club | 39.10 |