| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | GUITERREZ-ROBLES, Fabiola | Hardee |
34.50 | Avon Park Chet Brojek Track Invitational |
| 3 | JACKSON, Alexandra | Hardee |
27.69 | Bob English Invitational 2026 |
| 4 | MARGARITO-GOMEZ, Elizabeth | Hardee |
33.82 | Avon Park Chet Brojek Track Invitational |
| 5 | RAMON, Sophia | Hardee |
36.59 | Lake Placid Invitational 2026 |