| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | SANTIAGO, Diego | Hardee |
28.16 | Avon Park Chet Brojek Track Invitational |
| 2 | HEDGECOCK, Joshua | Hardee |
26.71 | Larry Richardson Memorial Invite |
| 3 | WATSON, Dariyon | Hardee |
22.62 | Avon Park Red Devil Invitational |
| 4 | PLEGER, Maverick | Hardee |
26.44 | Lake Placid Invitational 2026 |
| 5 | DICKSON, John Mason | Hardee |
27.41 | Lake Placid Invitational 2026 |