| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Sebring |
A | 11:28.43 | Blue Devil Invitational (Regio |
| 2 | Hardee |
A | 12:00.11 | Avon Park Meet #1 2025 |
| 3 | DeSoto County |
A | DNS | Avon Park Meet #1 2025 |
| 4 | Avon Park |
A | 13:21.03 | Lake Placid Invite |
| 5 | Lake Placid |
A | NT |