| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Hardee |
A | 8:46.73 | Larry Richardson Memorial Invitational |
| 2 | Avon Park |
A | 9:42.40 | Sebring Relay Meet 2025 |
| 3 | Sebring |
A | 9:45.00 | |
| 4 | DeSoto County |
A | 9:56.71 | Dunbar Tiger Classic Invite |
| 5 | Mulberry |
A | DNS | Frostproof - The Ridge Open 2025 |
| 6 | Lake Placid |
A | 10:14.36 | Lake Placid Invite |