| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | HAYS, Elle | Glades Day |
2:02.63 | Pahokee Andre Powell Invitational |
| 3 | BAEZ, Marina | Glades Day |
1:34.65 | Pahokee Anquan Boldin Invitational 2025 |
| 4 | BYTHWOOD, De'Asia | Pahokee |
1:24.32 | Jeff Fossen Invitational 2025 |
| 5 | JOHNSON, Kalyn | Clewiston |
1:24.95 | 65th Annual Edison Relays |
| 6 | BREDWOOD, Cambria | Clewiston |
1:45.92 | Lake Placid Invite |
| 7 | FIELDS, Tyra | Clewiston |
NT | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | PIERCE, Kaniah | Glades Central |
1:23.03 | PBGHS Invitational 2025 |
| 2 | HOLMES, Dallas | Pahokee |
1:22.06 | John I Leonard Invitational |
| 3 | SELLERS, Te'Azia | Glades Central |
1:19.06 | PBGHS Invitational 2025 |
| 4 | PORTER, Heraje | Royal Palm Beach |
1:14.17 | Pahokee Andre Powell Invitational |
| 5 | WOODSON, Xiomara | Pahokee |
1:18.02 | Pahokee Anquan Boldin Invitational 2025 |
| 6 | FOWLER, Ta'Niyah | Glades Central |
1:21.33 | Pahokee Andre Powell Invitational |
| 7 | HART, Alniyah | Pahokee |
1:22.33 | Jeff Fossen Invitational 2025 |
| 8 | CAROTENUTO, Tessa-lynn | Glades Day |
1:23.68 | John I Leonard Invitational |