| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | PATILLO, RoDajah | Santaluces |
NT | 14.62 |
| 3 | BYTHWOOD, De'Asia | Pahokee |
20.99 | Pahokee Anquan Boldin Invitational 2025 |
| 4 | PIERCE, Kaniah | Glades Central |
19.92 | Pahokee Andre Powell Invitational |
| 5 | POOLE, Paylyn | Glades Day |
20.78 | PBGHS Invitational 2025 |
| 6 | BREDWOOD, Cambria | Clewiston |
22.37 | Kings Academy Varsity 2 |
| 7 | GORDON, Yoriah | Glades Day |
NT | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | HOLMES, Dallas | Pahokee |
19.81 | John I Leonard Invitational |
| 2 | MINUS, Lawrencia | Glades Central |
19.42 | Pahokee Andre Powell Invitational |
| 3 | PORTER, Heraje | Royal Palm Beach |
18.25 | PBGHS Invitational 2025 |
| 4 | HART, Alniyah | Pahokee |
17.59 | Pahokee Andre Powell Invitational |
| 5 | BAILEY, Denijah | Glades Day |
17.66 | PBGHS Invitational 2025 |
| 6 | FOWLER, Ta'Niyah | Glades Central |
18.41 | Pahokee Andre Powell Invitational |
| 7 | PINTO, Winnyia | Glades Central |
19.65 | Pahokee Andre Powell Invitational |
| 8 | CASSEUS, Shelliah | Santaluces |
19.84 | Royal Palm Beach Meet 2025 |