| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Glades Day |
A | Sturgis | Sisnett | Ocanas | Bailey | DNS | |
| 3 | John I. Leonard |
A | Augustin | Dorceus | toussaint | lapointe | 4:28.45 | Clyde Harris Invitational |
| 4 | Pahokee |
A | Hamilton | Johnson | Johnson | White | 4:07.74 | Royal Palm Beach Invitational 2026 |
| 5 | Royal Palm Beach |
A | Chapman | Davis | Bradwell | Porter | 4:19.56 | Pahokee Invitational 2026 |
| 6 | Glades Central |
A | Butts | Kyles | Tibo | Marshall | 4:56.69 | Pahokee Invitational 2026 |