| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Glades Central |
A | Goring | Paul | Samuel | Kendall | DNF | |
| 2 | Atlantic (Delray) |
A | Barrera | Horne | Martinez | St Fort | DNF | 43.12 |
| 3 | Royal Palm Beach |
A | Curry | Civil | Rolle | Williams | 43.81 | Pahokee Invitational 2026 |
| 4 | Pahokee |
A | Simms | Fitzgerald | Stinfort | Hill | 42.49 | Pahokee Invitational 2026 |
| 5 | Somerset Canyons |
A | Seraphin | David | Bouie | Oliver | 43.59 | St Andrews Varsity 3 |
| 6 | Forest Hill |
A | Graham | Grant | Josapha | Emerson | 44.91 | Clyde Harris Invitational |
| 7 | Glades Day |
A | Williams | Jones | McKelvin | Villarreal | DNS | |
| 8 | Inlet Grove |
A | Lorius | Burrows | Francis | Lewis | DNS |