| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Somerset Canyons |
A | Occean | Jean Mary | Garcon | Miller | 54.96 | St Andrews Varsity 3 |
| 3 | John I. Leonard |
A | barrett | castillo | Dorceus | toussaint | 54.32 | John I Leonard Invitational 1 |
| 4 | Pahokee |
A | Jones | Johnson | Washington | White | 50.25 | Royal Palm Beach Invitational 2026 |
| 5 | Glades Central |
A | Josinvil | Forbes | Tibo | Marshall | 51.17 | Royal Palm Beach Invitational 2026 |
| 6 | Glades Day |
A | Ocanas | Baez | Sturgis | Sisnett | 54.48 | Pahokee Invitational 2026 |