| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Atlantic (Delray) |
A | Horne | Pericles | Cadet | Marshall | NT | |
| 2 | John I. Leonard |
A | gerilus | Joseph | louis | nelson | 3:37.09 | Clyde Harris Invitational |
| 3 | Royal Palm Beach |
A | Curry | Civil | Bowleg | Greenfield | 3:29.80 | Royal Palm Beach Invitational 2026 |
| 4 | Pahokee |
A | Logan | Jones | Stinfort | Morales | 3:28.19 | Royal Palm Beach Invitational 2026 |
| 5 | Santaluces |
A | Zennot | Montelus | Sanon | Gerald | 3:28.36 | Clyde Harris Invitational |
| 6 | Glades Central |
A | Goring | Baptist | Paul | Georges | 3:36.66 | Pahokee Invitational 2026 |
| 7 | Glades Day |
A | Capalbo | Muniz | Hogan | Roman | DNS |