| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Royal Palm Beach | A | guillaume | morgan | tipton | dort | 45.19 | John I Leonard Invitational |
| 3 | Glades Central | A | Demps | Johnson | Stinfort | Stinfort | 43.52 | Antone Smith's MuckCity Invite 1023 |
| 4 | Clewiston | A | Ray | Campbell | Trimble | Davis | 42.12 | FHSAA 2A Region 3 |
| 5 | Pahokee | A | Williams | Jackson | Gilmore | Jenkins | DNF | Antone Smith's MuckCity Invite 1023 |
| 6 | Immokalee | A | Blanchard | Frazier | Jorcely | Angello | DNS | Immokalee Meet #2 |
| 7 | Glades Day | A | Vereen | Willis | Clark | Vallee | 45.33 | Kings Academy Invitational |