| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Pine School |
A | Leighty | Whitaker | Bush | Anderson | 4:18.81 | Jensen Beach Invitational 2026 |
| 3 | American Heritage (DB) |
A | Martin | Kobe | Cesar | Riegler | 3:38.52 | Panther Relays 2025 |
| 4 | A | Blake | Apollon | Walker | Rheingold | 3:30.14 | FHSAA 3A Region 4 | |
| 5 | B | Gillyard | Robinson | Gilchrist | Gustama | 3:30.14 | FHSAA 3A Region 4 | |
| 6 | American Heritage (DB) |
B | Kiyota Ayrosa | Rudikoff | Tyson | Masrieh | 4:05.00 |