| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Legacy High School | A | Desir | Pierre Louis | Montenegro | Heysquierdo | 5:08.69 | Jensen Beach Meet #2 |
| 3 | Fort Pierce Central |
A | Blue | Benjamin | Spence | Alexander | 4:27.76 | Cobra Relays 2026 |
| 4 | Centennial |
A | Miller | Webber | Schmook | Chery | 4:09.07 | Louie Bing Invitational |
| 5 | Lincoln Park |
A | Anderson | Brown | Sanchez-Appell | Brianvil | 4:24.42 | 2026 St. Lucie County Championships |
| 6 | St. Edward's |
A | Hebig | Callaghan | Storts | Perrin | 4:43.39 | Sebastian River 29th Bill Wilson Invitational 2026 |