| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Legacy High School | A | Gooden | Gooden | Scalise | Meant | 46.23 | Cobra Relays 2026 |
| 3 | Fort Pierce Central |
A | Wilder | Sanon | Blackmon | Brown | 44.49 | 22nd Annual Warrior Invitational 2026 |
| 4 | Centennial |
A | Benito | Johnson | Thomas | Raphael | 41.48 | Louie Bing Invitational |
| 5 | Port St. Lucie |
A | Davis | Tavarez | Joubert | Pata | 43.41 | 2026 St. Lucie County Championships |
| 6 | Martin County |
A | Oh | Allison | Ocampo | Ozuna | 44.95 | Jensen Beach Invitational 2026 |
| 7 | St. Edward's |
A | Williams | Gatzke | Perrin | Barker | 49.04 | Vero Beach Friday Night Spikes II (FHSAA Sanctioned) |