| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | St. Edward's |
A | Wallace | Perrin | Blackman | Ensio | 4:18.60 | Pineapple Cove MS Track 3 2026 |
| 3 | Fort Pierce Central |
A | Baker | Sanon | Curry | Brown | 3:29.84 | Cobra Relays 2026 |
| 4 | Centennial |
A | Benito | Johnson | Mair | Bertrand | 3:18.71 | Louie Bing Invitational |
| 5 | Port St. Lucie |
A | Mayberry | Barnwell | Pata | Davis | 3:28.50 | 2026 St. Lucie County Championships |
| 6 | Lincoln Park |
A | Cunningham | Parfait | Goldberg | Brianvil | 3:59.43 | Sebastian River 29th Bill Wilson Invitational 2026 |