| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | ANDERSON, Amelia | Martin County |
1:28.08 | Bulldog Invitational 2025 |
| 3 | HEBIG, Nahlia | St. Edward's |
1:22.26 | Benjamin Invite #2 2025 (Sanctioned) |
| 4 | CALLAGHAN, Cadence | St. Edward's |
1:17.55 | Benjamin Invite #2 2025 (Sanctioned) |
| 5 | GREEN, Eriyanna | Centennial |
1:18.69 | Cobra Relays 2025 |
| 6 | LANE, Raniyah | Fort Pierce Central |
1:25.47 | MCHS Fesh/Soph 2025 |
| 7 | FEQUIERE, Jada | Fort Pierce Central |
1:28.17 | MCHS Fesh/Soph 2025 |