| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | ANDERSON, Amelia | Martin County |
23.01 | Cobra Relays 2025 |
| 3 | CALLAGHAN, Cadence | St. Edward's |
20.44 | Benjamin Invite #2 2025 (Sanctioned) |
| 4 | GREEN, Eriyanna | Centennial |
19.59 | Cobra Relays 2025 |
| 5 | LANE, Raniyah | Fort Pierce Central |
20.03 | Cobra Relays 2025 |
| 6 | FEQUIERE, Jada | Fort Pierce Central |
21.23 | MCHS Fesh/Soph 2025 |