400-800-1200-1600
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Centennial | A | Lundgren | Morrison | Johnson | Eason | NT | |
| 2 | Treasure Coast | A | Swann | Moore | Spang | Moore | 16:40.45 | Cobra Relays - 2022 |
| 3 | Lincoln Park | A | Drawdy | Watson | Duffy | Lane | 14:23.88 | Cobra Relays - 2022 |
| 4 | Jensen Beach | A | McIntyre | Haley | Gozdz | Vancour | 14:21.28 | Cobra Relays - 2022 |
| 5 | Martin County | A | Chaney | Homer | Ewing | Miguel | 14:21.99 | Cobra Relays - 2022 |
| 6 | Sebastian River | A | Vecchione | Gibbs | Kish | Sadley | 15:07.58 | 2023 Merritt Island Relays |
| 7 | Fort Pierce Central | A | Williams | Walker | Brianvil | Moore | 16:47.71 | Cobra Relays - 2022 |
| 8 | John Carroll | A | Reed | Moore | Martinez | Butterfield | NT |