| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 4 | Chaminade-Madonna |
A | Adras | Harris | Rowe | Denis | 3:30.41 | FHSAA 1A District 15 |
| 5 | Northeast (OP) |
A | Campbell | Jones | Compere | Brown | DNS | BCAA North Central Qualifier |
| 6 | Coral Springs |
A | Wykpisz | Gentilhomme | Doreus | Sammuels | DNS | Sam Burley Hall of Fame Invitational |
| 7 | Hallandale |
A | Randolph | Johnson | Pierrelouis | Eddie | DNF |