| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Boca Raton |
A | Dobson | Taylor | Saint-Louis | Brutus | 53.04 | FHSAA 4A District 12 |
| 4 | Boone |
A | Rowe | Chambers | Murphy | Hardy | 52.16 | FHSAA 4A District 9 |
| 5 | Tohopekaliga |
A | Pagan | Francois | Diaz Monge | Martinez | 52.68 | FHSAA 4A District 9 |
| 6 | Lake Worth |
A | Henry | Williams | Vilmont | Greenwich | 53.06 | FHSAA 4A District 12 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Wellington |
A | Brooks | Carbocci | Lowe | Weir | DNF | FHSAA 4A District 11 |
| 2 | Martin County |
A | Proctor | Wilson | Kochis | Antenor | 51.65 | FHSAA 4A District 10 |
| 3 | Park Vista |
A | Fleuricin | Bavaro | Brown | Surtain | 50.42 | FHSAA 4A District 12 |
| 4 | Treasure Coast | A | Brown | Benjamin | Frederick | Sama | 50.06 | FHSAA 4A District 10 |
| 5 | Olympia |
A | Massey | Nimade-Bonsu | Rhoden | Coleman | 50.40 | FHSAA 4A District 9 |
| 6 | Jupiter |
A | Granda | Norzea | Monterroso | Vails | 51.21 | FHSAA 4A District 11 |
| 7 | Centennial |
A | Kinchloe | Jones | Plantin | Dorelus | 51.79 | FHSAA 4A District 10 |
| 8 | Spanish River |
A | Ulysse | Theissen | Durso | Main | 51.97 | FHSAA 4A District 12 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Osceola (Kissimmee) |
A | Shelton | Varnado | Bright | Strachen | 49.85 | FHSAA 4A District 10 |
| 2 | Palm Beach Gardens |
A | Collier | Odita | Perry | Mcgee | 49.48 | FHSAA 4A District 11 |
| 3 | Vero Beach |
A | Phinizee | Gibson | Evans | Christian | 48.43 | FHSAA 4A District 10 |
| 4 | Dr. Phillips |
A | Rene | Martin | Castell | Smith | 48.07 | FHSAA 4A District 9 |
| 5 | Lake Nona |
A | Bispo | Hadley | Turner | Morris | 48.29 | FHSAA 4A District 9 |
| 6 | Seminole Ridge |
A | Gordon | Touma | James | Garced | 49.17 | FHSAA 4A District 11 |
| 7 | Dwyer |
A | Crumbry | McClean | Bias | Mccalla | 49.51 | FHSAA 4A District 11 |
| 8 | Palm Beach Central |
A | Hendrix | Earle | Rejouis | Mindley | 49.94 | FHSAA 4A District 11 |