| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | SMOOT, Trinity | Wellington |
27.33 | FHSAA 4A District 11 |
| 2 | RAMIREZ, Sophia | Wellington |
26.91 | FHSAA 4A District 11 |
| 3 | MCCLEAN, Ianna | Dwyer |
26.63 | FHSAA 4A District 11 |
| 4 | JONES-RAMPEL, Ashanti | Boca Raton |
26.55 | FHSAA 4A District 12 |
| 5 | GARCED, Aliyah | Seminole Ridge |
26.58 | FHSAA 4A District 11 |
| 6 | SURTAIN, Abigail | Park Vista |
26.66 | FHSAA 4A District 12 |
| 7 | MCGEE, Shanetta | Palm Beach Gardens |
27.18 | FHSAA 4A District 11 |
| 8 | ENNIS, Makayla | Palm Beach Lakes |
27.61 | FHSAA 4A District 11 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | BISPO, Annabel | Lake Nona |
26.49 | FHSAA 4A District 9 |
| 2 | HOSKINS, Riyoko | Centennial |
26.43 | FHSAA 4A District 10 |
| 3 | REJOUIS, Ashley | Palm Beach Central |
26.03 | FHSAA 4A District 11 |
| 4 | BENJAMIN, Kalani | Treasure Coast | 25.98 | FHSAA 4A District 10 |
| 5 | OLIVEIRA, Nicole | Harmony |
25.99 | FHSAA 4A District 10 |
| 6 | PERRY, A'cian | Palm Beach Gardens |
26.20 | FHSAA 4A District 11 |
| 7 | MCCALLA, Sariyah | Dwyer |
26.46 | FHSAA 4A District 11 |
| 8 | GORDON, Dana | Seminole Ridge |
26.51 | FHSAA 4A District 11 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | AUGUSTIN, Ketteline | John I. Leonard |
25.80 | FHSAA 4A District 12 |
| 2 | MARTIN, Riley | Dr. Phillips |
25.69 | FHSAA 4A District 9 |
| 3 | OVERTON, Taylor Nicole | Boca Raton |
25.15 | FHSAA 4A District 12 |
| 4 | MORRIS, Emma | Lake Nona |
24.96 | FHSAA 4A District 9 |
| 5 | CASTELL, Peyton | Dr. Phillips |
25.00 | FHSAA 4A District 9 |
| 6 | WILDER, La'Neal | Fort Pierce Central |
25.38 | FHSAA 4A District 10 |
| 7 | RODRIGUEZ, Alondra | Cypress Creek |
25.70 | FHSAA 4A District 9 |
| 8 | BRIGHT, Zanaya | Osceola (Kissimmee) |
25.91 | FHSAA 4A District 10 |