| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Lake Worth |
A | Middleton | Charles | Braynen | Rogers | 46.69 | 46.69 Atlantic High School Informal Meet |
| 3 | Boca Raton |
A | Simouang | Wells | Buchanan | Bloomfield | 44.17 | 44.17 Cardinal Newman Invitational 2024 |
| 4 | West Boca Raton |
A | Spitz | Rosenthal | Caceres | Gonzales | 43.77 | 43.77 West Boca Raton Invitational |
| 5 | Spanish River |
A | Monde | Campbell | Lamas | Wallace | 43.79 | 43.79 Cardinal Newman Invitational 2024 |
| 6 | Park Vista |
A | Thelamond | Letts | Bennett | Garvin | 44.27 | 44.27 Jeff Fossen Invitational 2024 |