| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Pine School |
A | Galbraith | DeGeorge | Stringfellow | Ekstrom | 4:42.26 |
| 3 | Jupiter Christian |
A | Fielder | WAYCHOWSKY | Whittington | Schiralli | 4:36.47 |
| 4 | Pahokee |
A | Johnson | Johnson | White | Richardson | 4:00.87 |
| 5 | Benjamin School |
A | Woods | White | Lewis | Balistreri | 4:12.64 |
| 6 | John Carroll |
A | Butterfield | Fulmer | Moore | Ward | 4:37.16 |
| 7 | St. Edward's |
A | Perez | Hebig | Perrin | Campbell | 4:46.82 |