| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Pine School |
A | Devaney | Anderson | Sprague | Sprague | NT |
| 3 | Oxbridge Academy |
A | Kapp | Cano | Quasha | Burrows | 3:49.83 |
| 4 | Benjamin School |
A | Heilpern | Luiz | Luiz | Ryba | 3:21.90 |
| 5 | Pahokee |
A | Bryant | Bryant | Edward | Jones | 3:28.47 |
| 6 | Jupiter Christian |
A | Schiralli | FLORA | Evans | WEDA | 4:10.43 |