| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | CAMPBELL, Elizabeth | St. Edward's |
5:43.31 |
| 2 | FOSTER, Fiona | Benjamin School |
5:47.13 |
| 3 | KLEIN, Leah | Benjamin School |
5:54.56 |
| 4 | MUNIZ, MAYA | Jupiter Christian |
5:55.07 |
| 5 | HILL, Samantha | Benjamin School |
5:57.05 |
| 6 | BUTTERFIELD, Riley | John Carroll |
6:02.93 |
| 7 | GALBRAITH, Amelie | Pine School |
6:20.82 |
| 8 | PERRIN, Jacquelyn | St. Edward's |
6:21.92 |
| 9 | PETER, Isabella | St. Edward's |
6:34.70 |