| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 3 | 349 | NICHOLS, GABRIELLE | SO | Dwyer | NT | |
| 4 | 102 | TOUSSAINT, DONIKA | SR | Inlet Grove | NT | |
| 5 | 429 | NEGRON, AMARIS | SO | Dwyer | NT |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 2 | 513 | AUSTIN, LARIANA | FR | Atlantic (Delray) | NT | |
| 3 | 80 | BLANC, EIKA | FR | Inlet Grove | NT | |
| 4 | 303 | HAMILTON, ADRYANNA | SR | Palm Beach Gardens | 1:18.92 | Jupiter Preseason 2023 |
| 5 | 458 | GABAUD, JAHDAE | JR | Dwyer | NT | |
| 6 | 733 | PETIT-HOMME, JALAH | FR | Lake Worth Christian | NT | |
| 7 | 490 | WATERS, TAMIA | SR | Atlantic (Delray) | NT |