| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | WILLIAMS, Destyne | Sebring |
NT | 27.00 |
| 4 | SANCHEZ, Monica | Sebring |
NT | NT |
| 5 | WHITE, Calia | LaBelle |
NT | 28.5 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | LETHBRIDGE, Meghan | Sebring |
NT | NT |
| 2 | CORTES, Ana | Lake Placid |
NT | NT |
| 3 | VILLA, Maria | Hardee |
34.24 | Frostproof - The Ridge Open 2024 |
| 4 | TAYLOR, Tenaj | LaBelle |
33.28 | Lake Placid Meet #2 |
| 5 | JARVIS, Zarionna | DeSoto County |
33.48 | Lake Placid Meet #2 |
| 6 | ALLEN, Elizabeth | Sebring |
NT | NT |
| 7 | FERDINAND, Haizal | South Lake |
NT | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | CLAYTON, Jayla | Gateway (Fort Myers) |
31.54 | Guy Thomas Memorial Classic (FHSAA & NFHS Sanctioned) |
| 2 | HOLLIS, Jazmya | South Lake |
30.53 | Panther Invitational |
| 3 | DILLON, Mykala | Lake Placid |
30.31 | IMG Spring Break Invitational |
| 4 | WHEATLE, Asania | LaBelle |
29.74 | Lake Placid Meet #2 |
| 5 | JOHNSON, Shamera | Lake Placid |
30.26 | Lake Placid Meet #2 |
| 6 | NADASKAY, Madelyn | Hardee |
30.49 | Frostproof - The Ridge Open 2024 |
| 7 | ERSKINE, Breanna | Gateway (Fort Myers) |
30.63 | 3rd Annual Panther Invitational |
| 8 | PAVON, Annabella | Gateway (Fort Myers) |
32.54 | Dunbar Tiger Classic (Approved Sanctioned Meet) |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | SHORT, Miracle | Immokalee |
29.25 | Glade Central Invitational 2024 |
| 2 | WALKER, Teraje | Lake Placid |
28.84 | FHSAA 2A District 11 |
| 3 | MILLSAP, Saniyah | South Lake |
28.73 | Dr. Phillips Panther Invitational |
| 4 | WHITEHURST, Azaria | Fort Meade |
25.89 | 12th Annual Hawks on the Hill Invitational |
| 5 | ROBERTS, Asia | South Lake |
25.90 | FHSAA 4A District 4 |
| 6 | SANDOVAL, Valerie | LaBelle |
28.78 | Lake Placid Meet #1 |
| 7 | HOLLEY, Jaeda | Hardee |
28.93 | FHSAA 2A District 11 |
| 8 | REGISTRA, Lithania | Immokalee |
29.66 | Glade Central Invitational 2024 |