| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Sebring |
A | 11:16.25 | TFRRS 04/22/23 - 12:20.00 |
| 2 | Hardee |
A | 11:35.63 | Frostproof - The Ridge Open 2024 |
| 3 | South Lake |
A | 11:55.23 | Dr. Phillips Panther Invitational |
| 4 | LaBelle |
A | 12:03.94 | Lake Placid Meet #2 |
| 5 | DeSoto County |
A | 12:27.67 | FHSAA 2A Region 3 |
| 6 | Lake Placid |
A | 13:16.69 | Lake Placid Meet #2 |