| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | THOMPSON, Kash | LaBelle |
NT | NT |
| 4 | GAYMON, Kashaad | Gateway (Fort Myers) |
1:14.57 | 2024 South Fort Myers Wolfpack Invitational Sanctioned |
| 5 | LILLARD, Andres | South Lake |
1:25.08 | Dr. Phillips Panther Invitational |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | AMES, Haydon | DeSoto County |
1:11.61 | Lake Placid Meet #2 |
| 2 | POWERS, Tyler | DeSoto County |
1:09.33 | Lake Placid Meet #2 |
| 3 | ASHLEY, Caleb | Gateway (Fort Myers) |
1:08.04 | Guy Thomas Memorial Classic (FHSAA & NFHS Sanctioned) |
| 4 | PIERRE, Chandron | Immokalee |
58.10 | FHSAA 3A District 12 |
| 5 | THOMAS, Chacour | Gateway (Fort Myers) |
1:05.16 | 10th Annual Loretta Purish Invitational |
| 6 | MARTINEZ, Oscar | Gateway (Fort Myers) |
1:09.00 | Edison Relays 2024 (Approved Sanctioned Meet) |
| 7 | KINNETT, Jayden | South Lake |
1:10.41 | Dr. Phillips Panther Invitational |