| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | HENDERSON, E'Rajae | Gateway (Fort Myers) |
NT | NT |
| 4 | ZEPEDA, Fernando | DeSoto County |
23.24 | Lake Placid Meet #1 |
| 5 | GAYMON, Kashaad | Gateway (Fort Myers) |
NT | NT |
| 6 | RICHARDS, Kevon | South Lake |
NT | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | AMES, Haydon | DeSoto County |
21.53 | Lake Placid Meet #1 |
| 2 | POWERS, Tyler | DeSoto County |
20.58 | 2024 Hardee Wildcat Relay Meet |
| 3 | ASHLEY, Caleb | Gateway (Fort Myers) |
18.85 | Guy Thomas Memorial Classic (FHSAA & NFHS Sanctioned) |
| 4 | THOMAS, Chacour | Gateway (Fort Myers) |
16.62 | Guy Thomas Memorial Classic (FHSAA & NFHS Sanctioned) |
| 5 | BULLARD, JR., Douglas | Lake Placid |
17.15 | IMG Spring Break Invitational |
| 6 | KINNETT, Jayden | South Lake |
20.43 | Dr. Phillips Panther Invitational |
| 7 | THOMPSON, Kash | LaBelle |
21.37 | Lake Placid Meet #2 |
| 8 | DESIR, Isaiah | South Lake |
22.23 | Dr. Phillips Panther Invitational |