| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | WINFREY, Angelina | Hardee |
NT | NT |
| 4 | PADRON, Anahi | DeSoto County |
23.42 | Lake Placid Meet #2 |
| 5 | SMITH, Emily | LaBelle |
23.48 | Lake Placid Meet #1 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | MORGAN, Vanessa | DeSoto County |
22.88 | Lake Placid Meet #2 |
| 2 | ANTOINE, Abbigail | Gateway (Fort Myers) |
20.87 | Guy Thomas Memorial Classic (FHSAA & NFHS Sanctioned) |
| 3 | PIERRE, Danaelle | Hardee |
19.00 | George Jenkins - Bob English Invitational 2024 |
| 4 | BECKER, Paige | South Lake |
17.89 | Dr. Phillips Panther Invitational |
| 5 | GALETY, Caliphta | Gateway (Fort Myers) |
18.94 | Guy Thomas Memorial Classic (FHSAA & NFHS Sanctioned) |
| 6 | MICHEL, Keyshia | Gateway (Fort Myers) |
19.20 | FHSAA 2A District 12 |
| 7 | LOVINCE, Rakeysha | Gateway (Fort Myers) |
21.49 | 2024 South Fort Myers Wolfpack Invitational Sanctioned |