| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | South Fork |
A | Jackson-Fischer | Holmes | Pepsidero | Churchill | 52.66 | FHSAA 3A District 14 |
| 4 | Wellington |
A | Brooks | Carbocci | Weir | Ramirez | 51.94 | FHSAA 4A District 11 |
| 5 | Dreyfoos |
A | Mcdow | Vasallo | Pierre | Cannon | 52.05 | FHSAA 2A Region 4 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Park Vista |
A | Morales | Haldas | Brown | Collins | 50.42 | FHSAA 4A District 12 |
| 3 | Dwyer |
A | Nichols | DuVal | Gil | Perry | 49.45 | Palm Beach County Championships |
| 4 | American Heritage (DB) |
A | Milord | Watts | Cage | Johnson | 48.63 | Bob Hayes High School Invitational |
| 5 | Palm Beach Central |
A | Turner | Ross | Henry | Earle | 49.39 | FHSAA 4A Region 3 |
| 6 | Martin County |
A | Edwards | Perez | Garcia | Antenor | 49.64 | FHSAA 4A Region 3 |
| 7 | King's Academy |
A | Raines | Brabham | Esquivel | Cousins | 51.10 | FHSAA 2A District 13 |