| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Lincoln Park |
A | Sena | Rosichan Nader | Louis | Parfait | NT | |
| 4 | Fort Pierce Westwood |
A | Boone | Ford | Lynch | Owens | DNS | |
| 5 | John Carroll |
A | Monaco | Summerall | Perez | Bradley | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Tradition Prep | A | Demosthenes | Quintavalle | Frederic | Riveron | 3:56.09 | Bulldog Invitational 2025 |
| 2 | Martin County |
A | Allison | Ineus | St. Juste | Eysaman | 3:41.09 | Bulldog Invitational 2025 |
| 3 | Vero Beach |
A | Jacobs | Powell | Lewis | 3:32.03 | Holy Trinity Invitational | |
| 4 | Fort Pierce Central |
A | Ransfer | Nathan-Marsh Jr | Deleon | Beauford | 3:24.00 | |
| 5 | Centennial |
A | Johns | Enoch | Polynice | Jean-Pierre | 3:27.17 | Bill Wilson Invitational 2025 |
| 6 | Treasure Coast | A | Beausejour-Joseph | Harper | Jones | Reed | 3:34.91 | Park Vista #1 2025 |
| 7 | Sebastian River |
A | Osorio Espitia | Sapp | Lauro | Carilus | 3:46.56 | Holy Trinity Invitational |