| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Tradition Prep | A | Falcon | Phillippe | Hamilton | Chavers | NT | |
| 4 | Lincoln Park |
A | Lundy | Huskin | Brown | Anderson | NT | |
| 5 | Martin County |
A | Perez | Ebanks | Osdon | Garcia | DNS | |
| 6 | Treasure Coast | A | Smith | Brown | Labbe | Delevery | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Fort Pierce Westwood |
A | Brown | Angervil | Figaro | Holmes | NT | |
| 3 | Sebastian River |
A | Gibson | Wiggins | Walters | Carilus | 1:51.15 | Bill Wilson Invitational 2025 |
| 4 | Fort Pierce Central |
A | Andrews | Bolden | Lane | Wilder | 1:32.00 | |
| 5 | Vero Beach |
A | Gibson | Ewar | Wells | Overton | 1:49.61 | Bill Wilson Invitational 2025 |
| 6 | Centennial |
A | Schmook | Hoskins | Plantin | Mitton | 1:58.59 | Bill Wilson Invitational 2025 |