| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Treasure Coast | A | Harper | Isaac | Jones | Richardson | NT | |
| 3 | Martin County |
A | Dixon | Kideys | McLean | Ineus | DQ | |
| 4 | John Carroll |
A | Coleman | Summerall | Johnson | Smith | NT | |
| 5 | Lincoln Park |
A | Rosichan Nader | Rosichan Nader | Eshleman | Holland | NT | |
| 6 | Tradition Prep | A | Richardson | Heredia | Lopez | Drummond | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Fort Pierce Westwood |
A | Lynch | Mckenzie | Ford | Roberts | NT | |
| 3 | Sebastian River |
A | Gibbs | White | St. John | Smith | 1:37.95 | Bill Wilson Invitational 2025 |
| 4 | Fort Pierce Central |
A | Ransfer | Hills | Reynolds | Saint-Jean | 1:20.00 | |
| 5 | Centennial |
A | Johns | Jean-Pierre | Enoch | Polynice | 1:33.93 | Bill Wilson Invitational 2025 |
| 6 | Vero Beach |
A | Lewis | Stinson | Trusty | Jacobs | DQ | Bill Wilson Invitational 2025 |