| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | John Carroll |
A | Martinez | Nduku | Delion | Delince | NT | |
| 4 | Tradition Prep | A | Hamilton | Penarand | Falcon | Chavers | 55.90 | Bulldog Invitational 2025 |
| 5 | Fort Pierce Westwood |
A | Angervil | Brown | Williams | Figaro | NT | |
| 6 | Lincoln Park |
A | Huskin | Lundy | Brown | Bacon | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Treasure Coast | A | Brown | Labbe | Smith | Sama | 52.42 | Bulldog Invitational 2025 |
| 3 | Sebastian River |
A | McCray | James | Bradley | Narcisse | 51.10 | Holy Trinity Invitational |
| 4 | Vero Beach |
A | Tomlin | Ewar | Lundy | Overton | 47.77 | Bill Wilson Invitational 2025 |
| 5 | Fort Pierce Central |
A | Lane | Wilder | Andrews | Bolden | 48.00 | |
| 6 | Centennial |
A | Sinclair | Hoskins | Robinson | Schmook | 51.13 | Bill Wilson Invitational 2025 |
| 7 | Martin County |
A | Edwards | Fernandes | Perez | Ebanks | 53.99 | Dr Garcia Invite #1 2025 |