| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Lincoln Park |
A | NT | |
| 3 | Fort Pierce Westwood |
A | 2:08.80 | MCHS Tiger Relays |
| 4 | Centennial |
A | 1:54.20 | MCHS Tiger Relays |
| 5 | St. Edward's |
A | 2:06.20 | MCHS Tiger Relays |
| 6 | Tradition Prep | A | NT | |
| 7 | Fort Pierce Central |
A | NT |