| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Palm Beach Central |
A | Marseille | Penegrass | Ledford | Brown | DNS | Welington Meet #1 |
| 4 | Cardinal Newman |
A | Gantt | Elam | Holland | Mosley | 41.34 | Harry Howell Invitational Hosted by Cardinal Newman (Qualifi |
| 5 | Cardinal Newman |
B | Charles | Hart | Newby | Mcclellion | 41.34 | Harry Howell Invitational Hosted by Cardinal Newman (Qualifi |