| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | CASSEUS, Shelliah | Santaluces |
19.52 | Glades Central Invite 2026 |
| 4 | DAWKINS, Isabel | Cardinal Newman |
15.59 | Palm Beach Gardens Invite #1 2026 |
| 5 | PATILLO, RoDajah | Santaluces |
16.31 | Glades Central Invite 2026 |
| 6 | LOUIS, jenny | John I. Leonard |
21.67 | Pahokee Invitational 2026 |