| Lane | # | Athlete | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | 705 | STOKES III, John | Power House Athletics Track & | 2:30.00 | |
| 2 | 720 | DANIEL, Elijah | Running Tigers Track Club | NT | |
| 3 | 643 | DOSS, Dillon | Hillsborough Harriers | NT | |
| 4 | 650 | MAUGHAN, James | Hillsborough Harriers | NT | |
| 5 | 809 | NOEL, Marcus | Trailblazers Youth Athletic Mi | NT | |
| 6 | 658 | ROSE, Jaxson | Hillsborough Harriers | NT | |
| 7 | 787 | WHITE, Kamari | St Pete Nitro | NT |