| Lane | # | Athlete | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | 432 | HUGHES, Kendalle | Davinci Speed | NT | |
| 2 | 718 | CUASAY, Addison | Speed Starz Track/Running Club | NT | |
| 3 | 755 | ARCIDIACONO, Amelea | Unattached | 1:12.00 | |
| 4 | 596 | SMITH, Lentajhae | 352 Elite | 59.00 | |
| 5 | 772 | KLAUS, Chanine | VAST Track & Field and Cross C | 1:04.00 | |
| 6 | 594 | RANDAZZO, Jasmine | 352 Elite | NT | |
| 7 | 723 | PILCHER, London | Speed Starz Track/Running Club | NT |